Journal article
A semi-automated pipeline for quantifying drusen-like deposits in human induced pluripotent stem cell-derived retinal pigment epithelium cells
Jenna Hall, Maciej Daniszewski, Shane Cheung, Kalyan Shobhana, Himeesh Kumar, Helena H Liang, Henry Beetham, Ellie Cho, Carla Abbott, Alex W Hewitt, Kaylene J Simpson, Robyn H Guymer, Daniel Paull, Alice Pebay, Grace E Lidgerwood
SLAS Technology | Elsevier | Published : 2024
Abstract
Age-Related Macular Degeneration (AMD) is a highly prevalent form of retinal disease amongst Western communities over 50 years of age. A hallmark of AMD pathogenesis is the accumulation of drusen underneath the retinal pigment epithelium (RPE), a biological process also observable in vitro. The accumulation of drusen has been shown to predict the progression to advanced AMD, making accurate characterisation of drusen in vitro models valuable in disease modelling and drug development. More recently, deposits above the RPE in the subretinal space, called reticular pseudodrusen (RPD) have been recognized as a sub-phenotype of AMD. While in vitro imaging techniques allow for the immunostaining o..
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Grants
Awarded by National Health and Medical Research Council (NHMRC) Synergy grant
Awarded by Medical Research Future Fund-Stem Cell Therapies Mission grant
Awarded by NHMRC Senior Research Fellowship
Awarded by Australian Research Council (ARC) Training Centre for Personalised Therapeutics Technologies
Funding Acknowledgements
This research was supported by a National Health and Medical Research Council (NHMRC) Synergy grant (1181010, RHG, AP) , co -led by the RPD Consortium principle investigators and collaborators (see Appendix 1) . The authors acknowledge the facilities and the scientific and technical assistance of the iPSC reprogramming facilities (MD, AP) , the Stem Cell Disease Modelling laboratory, the University of Melbourne, which is supported by Phenomics Australia (PA) through funding from the Australian Government's National Collaborative Research Infrastructure Strategy (NCRIS) program. The iPSC lines were generated with support from PA. Confocal Imaging was performed at the Biological Optical Microscopy Platform (BOMP) Facility at The University of Melbourne. Further research support was provided by a Medical Research Future Fund-Stem Cell Therapies Mission grant (MRF1200678, KS, AWH, RHG, AP, GEL) ; the DHB Foundation (GEL, AP) ; a NHMRC Senior Research Fellowship (AP, 1154389) ; the Australian Research Council (ARC) Training Centre for Personalised Therapeutics Technologies (IC170100016) ; the University of Melbourne and Operational Infrastructure Support from the Victorian Government. The Victorian Centre for Functional Genomics (KJS) is funded by the Australian Cancer Research Foundation (ACRF) , the Peter MacCallum Cancer Centre Foundation and the University of Melbourne Collaborative Research Infrastructure Program. The Centre for Eye Research Australia receives support through the Victorian Government Operational Instructure program.